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Olive scab or leaf spot, Spilocaea oleagina

Olive scab or leaf spot, caused by the fungus Spilocaea oleagina, is widespread in the Mediterranean region. Losses arise mostly from defoliation of severely infected trees, with consequently reduced yield.

Symptoms

Symptoms are mainly confined to leaves and appear as dark brown, circular, zonate spots surrounded by yellow haloes ('peacocks eye'). S. oleagina shows a typical subcuticular growth, forming flat colonies within the cutinized layer of the thick epidermal cell wall. This habit has been associated with a defence reaction of the host involving mobilization and breakdown of the phenolic glucoside oleuropin and inhibition of pectolytic enzymes produced by the pathogen. The disease is particularly severe in densely planted groves of susceptible olive cultivars and in nurseries. Infections may occur throughout the year, except during hot and dry summers, when favourable temperatures (opt. 16–21°C) and rain occur. Conidia, formed at the apex of short ampulliform conidiophores, are usually carried by rain droplets, but recent data show that humid air currents and insects also contribute to limited aerial dissemination. Usually, the incubation period is about 2 weeks; however, if infection is followed by a hot season, it may last several weeks. Spots already formed in spring may stop growing in summer and resume their growth and sporulation in autumn. Chemical control schedules include fungicide (especially copper) treatments during the main infection seasons (spring and autumn).

Disease Life Cycle

Heavily infected leaves and fruits on the ground and, as well as on those that remained on the tree, are a source of inoculum for the current season or allow over-wintering of the fungus. The pathogen is known generally to survive unfavourable conditions, e.g. dry, hot weather, in fallen leaves as well as in infected leaves on the tree. The conidia formed in leaves on the tree can survive for several months; although once they have separated from the conidiophores they lose their germination ability in less than a week (Viruega and Trapero 1999). Following a period of moist warm weather new batches of conidia are readily produced on the foliar spots. Viable conidia are also produced in fallen leaves. However, their role as inoculum to produce new infections is considered to be negligible (Trapero and Blanco 2008). This study has shown that the disease generally is favoured by cool weather; however, the warm and humid weather during the summer of 2009 was also observed to encouraged disease occurrence. These observations support those of Viruega and Trapero (1999), who found that in Spain leaf infections can remain latent over summer without causing any leaf drop and are the main source of inoculum for autumn–winter infection. Observations also indicated that young leaves were very susceptible to infection in spring, and that foliage in lower parts of trees was more frequently infected. This is consistent with the pathogen’s requirement for high humidity to develop. Germination requires 98% humidity, with temperatures in the range of 0–27°C (Trapero Cassas 1994). In Australia the disease can be inactive during the hot and dry summers. Germination of the spores is restricted at temperatures above 30°C. The percentage of germinating conidia decreases linearly in proportion to leaf age, being 58% at 2 weeks and 35% at 10 weeks. Temperature significantly affects the frequencies of conidium germination on wet leaves from 5°C to 25°C. The percent germination increases from 16.1, 23.9, 38.8 to 47.8 and decreases again to 35.5% after 24 h. Formation of appressoria occurred 6 h after the first signs of germination. The percentage of germlings with appressoria increases with increasing temperature to a maximum of 43% at 15°C. No appressoria is formed at 25°C after 48 h of incubation. Increasing wetness duration causes increasing numbers of conidia to germinate at all temperatures tested (5–25°C). The minimum leaf wetness periods required for germination at 5, 10, 15, 20 and 25°C were 24, 12, 9, 9 and 12 h, respectively. At 20°C, a shorter wetness period (6 h) is sufficient if germinating conidia were then placed in 100% RH, but not at 80 or 60%. However, no conidia germinates without free water even after 48 h of incubation at 20°C and 100% RH. The graphical presentation of the Peacock spot model shows the leaf wetness, relative hiumidity and air temperature together with the results for infection progress and infection severity. The graph below indicates an infection starting at December 17th at 19:00 and finishing by 100% at December 18th at 11:00. The infection severity is calculated after a longer moist period and it increases in steps to 4, what means the highest calculated severity for this infection period.

References

Graniti A (1993) Olive scab: a review. OEPP/EPPO Bulletin 23, 377–384. MacDonald AJ, Walter M, Trought M, Frampton CM, Burnip G (2000) Survey of olive leaf spot in New Zealand. New Zealand Plant Protection 53, 126–132.
Obanor EO, Walter M, Jones EE, Jaspers MV (2005) In vitro effects of fungicides on conidium germination of Spilocaea oleagina, the cause of olive leaf spot. New Zealand Plant Pathology 58, 278–282.
Schubert K, Ritschel A, Braun U (2003) A monograph of Fusicladium s.lat. (Hyphomycetes). Schlechtendalia 9, 71–132.
Shabi E, Birger R, Lavee S, Klein I (1994) Leaf spot (Spilocaea oleaginea) on olive in Israel and its control. Acta Horticulturae 356, 390–394.
Trapero Cassas A (1994) El repilo del olivo. Agricultura 746, 788–790.
Trapero A, Blanco MA (2008) Enfermedades. pp. 557–614. In ‘El cultivo de olivo. 6th edition.’ (Eds D Barranco, R Fernández-Escobar, L Rallo) 846 pp. (Coedición Junta de Andalucía/Mundi-Prensa: Madrid, Spain)
Viruega JR, Lique F, Trapero A (1997) Caída de aceituhas debida a infectciones del pedunculo por Spilocaea oleagina, agente del Repilo del olivo. Fruticultura Profesional 88, 48–54.
Viruega JR, Trapero A (1999) Epidemiology of leaf spot of olive tree caused by Spilocaea oleagina in southern Spain. Acta Horticulturae 474, 531–534.